[JaICA] Highly Sensitive 8-OHdG Check ELISA

[JaICA 한국공식대리점]

[JaICA] Highly Sensitive 8-OHdG Check ELISA

Specifications

• Assay principle: Competitive ELISA (detection: 450 nm)
• Specifity: Specific for 8-OHdG. Antibody have been tested to 8-OHdG analogues (guanosine(G),7-methyl-G, 6-SH-G, 8-Bromo-G, dA, dC, dT, dI, dU, dG, O6-methyl-dG,8-OHdA, guanine(Gua),O6-methyl-Gua, 8-OH-Gua, uric acid, urea, creatine, creatinine, 8-sulfhydryl-G, 8-OH-G).
• Measuring range: 0.125 to 10 ng/mL
• Format: 96 wells (18 samples in triple assays)
• Applications: Urine, serum, tissue and cultured cells.
• Storage: Store at 4 - 10°C (don't freeze).
• Expiry: 9 months after the day of manufacturing.
• Required but not provided:
  - Micropipet and chip (100 µL, 1000 µL).
  - Measuring pipet (10 mL, 20 mL)/ measuring cylinders.
  - 8 or 12-syncronous multichannel pipet and reagent tray for multichannel pipet.
  - Microplate reader (filter; 450 nm).

 

References

1) H.Kasai, P.F.Crain, Y.Kuchino, S.Nishimura,A.Ootsuyama and H.Tanooka : Formation of 8-hydroxyguaine moiety in cellular DNA by agents producing oxygen radicals and evidence for its repair. Carcinogenesis 7(11), p1849-1851 (1986) [ 8-OHdG/8-OHG is formed by reactive oxygen radicals. ]

2) R.G.Cutler: Antioxidants and aging. Am J Clin Nutr 50,p373S-379S (1991)
[ Relationship between lifespan and antioxidants, enzymes and 8-OHdG. ]

3) S.Toyokuni, T.Tanaka, Y.Hattori, Y,Nishiyama, A.Yoshida, K.Uchida, H.Hiai, H.Ochi and T.Osawa: Quantitative immunohistochemical determination of 8-hydroxy-2'deoxyguanosine by a monoclonal antibody N45.1: Its application to ferric nitrilotriacetate-induced renal carcinogenesis model. Lab Invest 76(3), p365-374 (1997) [ Specifity of the antibody clone N45.1.]

4) Saito S, Yamauchi H, Hasui Y, Kurashige J, Ochi H, Yoshida K: Quantitative determination of urinary 8-hydroxydeoxyguanosine (8-OH-dg) by using ELISA. Res Commun Mol Pathol Pharmacol 107(1-2),p39-44 (2000)[ Development and assessment of 8-OHdG Check ELISA. ]

5) M.D.Evans, M.S.Cooke, I.D.Podmore, Q.Zheng, K.E.Herbert and J.Lunec: Discrepancies in the measurement of UVC-induced 8-oxo-2'-deoxyguanosine: Implications for the analysis of oxidative DNA damage. Biochem Biophys Res Commun 259, p374-378 (1999)
[ ELISA results show high correlation between those of HPLC-ECD when 8-OHdG in DNA samples are assessed. ]

6) Ha Won Kim, Akira Murakami, Marshall V Williams and Hajime Ohigashi: Mutagenicity of reactive oxygen and nitrogen species as detected by co-culture of activated inflammatory leukocytes and AS52 cells. Carcinogenesis 24(2), p235-241 (2003)[ Detection of 8-OHdG in cultured cells. ]

7) Watanabe E, Matsuda N, Shiga T, Kajimoto K, Ajiro Y, Kawarai H, Kasanuki H, Kawana M: Significance of 8-hydroxy-2'-deoxyguanosine levels in patients with idiopathic dilated cardiomyopathy. J Card Fail. 2006 Sep;12(7):527-32.[ Serum 8-OHdG in IDC patients are assessed. ]

8) Shiihara T, Kato M, Ichiyama T, Takahashi Y, Tanuma N, Miyata R, Hayasaka K: Acute encephalopathy with refractory status epilepticus: Bilateral mesial temporal and claustral lesions, associated with a peripheral marker of oxidative DNA damage. J Neurol Sci. 250(1-2):159-61(2006)
[ Measurement of 8-OHdG in human urine, serum and cerebro spinal fluid(CSF). ]

9) Reiko Nagasaka, Nobuaki Okamoto, Hideki Ushio: Effects of caloric restriction on post-spawning death of ayu. Exp Gerontol 40, p556-561(2005)[ Detection of 8-OHdG in fish tissues (brain and liver). ]

10) Yasuda M, Ide H, Furuya K, Yoshii T, Nishio K, Saito K, Isotani S, Kamiyama Y, Muto S, Horie S: Salivary 8-OHdG: a useful biomarker for predicting severe ED and hypogonadism. J Sex Med. 5(6),p1482-1491(2008)[ 8-OHdG in saliva can be detected by 8-OHdG ELISA. ]
 

Product name	Code	Assay range	Application
Highly Sensitive 8-OHdG Check	KOG-HS10E	0.125 - 10 ng/mL	Serum, Plasma, tissue and other biological fluids

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